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ELK Biotechnology

EM1029

β-Tubulin Mouse Monoclonal Antibody

β-Tubulin Mouse Monoclonal Antibody

$248.00 USD
$248.00 USD
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β-Tubulin Mouse Monoclonal Antibody

Overview

Product name: β-Tubulin Mouse mAb
Reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit,Sheep, Insect, Yeast
Alternative Names: N/A
Source: Mouse
Applications: WB, IHC, IF
Species: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit,Sheep, Insect, Yeast
Species: Human
Recommended dilutions: WB 1:5,000 IHC 1:200 IF 1:100-200
Immunogen: Synthetic Peptide
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/ml
Observed band: 55kDa
GeneID（Human）: 203068
Human Swiss-Prot No: P07437
Cellular localization: Cytoplasm,Cytoskeleton,Microtubule
Background: Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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