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ELK Biotechnology
SKU:EM1028
GAPDH Mouse Monoclonal Antibody
GAPDH Mouse Monoclonal Antibody
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$248.00 USD
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$248.00 USD
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GAPDH Mouse Monoclonal Antibody
Overview
| Product name: | GAPDH Mouse mAb |
| Reactivity: | Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast |
| Alternative Names: | N/A |
| Source: | Mouse |
| Applications: | WB, IHC, IF |
| Species: | Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast |
| Species: | Human |
| Recommended dilutions: | WB 1:5,000 IHC 1:200 |
| Immunogen: | Synthetic Peptide |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | 37kDa |
| GeneID(Human): | 2597 |
| Human Swiss-Prot No: | P04406 |
| Cellular localization: | Cytoplasm,Cytoskeleton,Membrane,Nucleus |
| Background: | Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. |
Precision
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
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