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ELK Biotechnology

EM1024

Flag-Tag(HRP Conjugated) Mouse Monoclonal Antibody

Flag-Tag(HRP Conjugated) Mouse Monoclonal Antibody

$248.00 USD
$248.00 USD
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Flag-Tag(HRP Conjugated) Mouse Monoclonal Antibody

Overview

Product name: Flag-Tag(HRP Conjugated) Mouse mAb
Reactivity: N/A
Alternative Names: DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag
Source: Mouse
Applications: WB
Species: N/A
Species: N/A
Recommended dilutions: WB 1:5,000-10,000
Immunogen: Synthetic Peptide
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/ml
Observed band: N/A
GeneID（Human）: N/A
Human Swiss-Prot No: N/A
Cellular localization: N/A
Background: The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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