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ELK Biotechnology

SKU:EM1005

VSV-G-Tag Mouse Monoclonal Antibody

VSV-G-Tag Mouse Monoclonal Antibody

Regular price $248.00 USD
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VSV-G-Tag Mouse Monoclonal Antibody

Overview

Product name: VSV-G-Tag Mouse mAb
Reactivity: N/A
Alternative Names: N/A
Source: Mouse
Applications: WB
Species: N/A
Species: N/A
Recommended dilutions: WB 1:5,000 IP 1:200 IF 1:1,000
Immunogen: Synthetic Peptide
Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Clonality: Monoclonal
Isotype: IgG1
Concentration: 1mg/ml
Observed band: N/A
GeneID(Human): N/A
Human Swiss-Prot No: N/A
Cellular localization: N/A
Background: Vesicular stomatitis virus (VSV), an enveloped RNA virus from the Rhabdoviridae family, is released from the plasma membrane of host cells by a process called budding. The fusiogenic envelope G glycoprotein of the vesicular stomatitis virus (VSV-G) that has been used to pseudotype retrovirus and lentivirus vectors can be used alone as an efficient vehicle for gene transfer. VSV-G protein is secreted into the culture medium as sendimentable vesicles from cells transfected with a VSV-G expression plasmid in the absence of other viral components. The VSV-G vesicles in the conditioned medium can be partially purified by pelleting through sucrose cushion ultracentrifugation.

Precision

Intra-assay Precision (Precision within an assay):CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays):CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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