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ELK Biotechnology
SKU:EM1003
His-Tag Mouse Monoclonal Antibody
His-Tag Mouse Monoclonal Antibody
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$248.00 USD
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His-Tag Mouse Monoclonal Antibody
Overview
| Product name: | His-Tag Mouse mAb |
| Reactivity: | N/A |
| Alternative Names: | N/A |
| Source: | Mouse |
| Applications: | WB, IP, IF |
| Species: | N/A |
| Species: | N/A |
| Recommended dilutions: | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Immunogen: | Synthetic Peptide |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | N/A |
| GeneID(Human): | N/A |
| Human Swiss-Prot No: | N/A |
| Cellular localization: | N/A |
| Background: | A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. |
Precision
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
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IP antibody use:5ug His Mouse IgG1 per ml Lysate,WB 1:3000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with His-tag fusion protein 3、IP (untransfected 293+anti-His mAb+ Protein G agarose) 4、IP (transfected 293+ normal Mouse IgG+Prote -
IF analysis of 293 cells transfected with a His-tag protein, using ELKbio anti-HisTag Mouse mAb at a 1:1000 dilution (blue DAPI, red anti-His) -
2ug His fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000 -
1、Multi-Tag Recombinant protein 2、293T transfected with His recombinant protein 3、Insect cell trandfected with His recombinant protein
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