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ELK Biotechnology
SKU:EM1001
C-Myc-Tag Mouse Monoclonal Antibody
C-Myc-Tag Mouse Monoclonal Antibody
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$248.00 USD
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C-Myc-Tag Mouse Monoclonal Antibody
Overview
| Product name: | C-Myc-Tag Mouse mAb |
| Reactivity: | N/A |
| Alternative Names: | N/A |
| Source: | Mouse |
| Applications: | WB, IP, IF |
| Species: | N/A |
| Species: | N/A |
| Recommended dilutions: | WB 1:5,000 IP 1:200 IF 1:1,000 |
| Immunogen: | Synthetic Peptide |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | N/A |
| GeneID(Human): | N/A |
| Human Swiss-Prot No: | N/A |
| Cellular localization: | N/A |
| Background: | c-Myc-tag antibody is part of the Tag series of antibodies, the best quality in the research. Myc protein is a transcription factor that activates expression of a great number of genes through binding on consensus sequences (Enhancer Box sequences (E-boxes)) and recruiting histone acetyltransferases (HATs). A recent study demonstrated that temporary inhibition of Myc selectively kills mouse lung cancer cells, making it a potential cancer drug target. |
Precision
Intra-assay Precision (Precision within an assay):CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
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IP antibody use:3ug Flag Mouse IgG1 per ml Lysate,WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with C-Myc-tag fusion protein 3、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4、IP (transfected 293+anti- C-Myc mAb+ P -
1ug C-Myc fusion protein+ Primary antibody dilution at 1、1:5,000 2、1:10,000 -
IF analysis of 293 cells transfected with a C-Myc-tag protein, using ELKbio anti-C-MycTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-C-Myc)
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