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ELK Biotechnology
SKU:EM1000
Flag-Tag Mouse Monoclonal Antibody
Flag-Tag Mouse Monoclonal Antibody
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$248.00 USD
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Flag-Tag Mouse Monoclonal Antibody
Overview
| Product name: | Flag-Tag Mouse mAb |
| Reactivity: | N/A |
| Alternative Names: | N/A |
| Source: | Mouse |
| Applications: | WB, IP, IF |
| Species: | N/A |
| Recommended dilutions: | WB 1:5,000 IP 1:200 IF 1:2,000 |
| Immunogen: | Synthetic Peptide |
| Storage: | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles. |
| Clonality: | Monoclonal |
| Isotype: | IgG1 |
| Concentration: | 1mg/ml |
| Observed band: | N/A |
| GeneID(Human): | N/A |
| Human Swiss-Prot No: | N/A |
| Cellular localization: | N/A |
| Background: | The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence. |
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IP antibody use:5ug Flag Mouse IgG1 per ml Lysate,WB 1:5000 1、untransfected 293 cell lysate 2、transfected 293 cell lysate with Flag-tag fusion protein 3、IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4、IP (transfected 293+anti- Flag mAb+ P -
1ug Flag fusion protein+ Primary antibody dilution at 1:10,000. -
IF analysis of 293 cells transfected with a Flag-tag protein, using ELKbio anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag)
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