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ELK Biotechnology

ELK9353

Human Krebs Von den Lungen-6(KL-6) ELISA Kit

Human Krebs Von den Lungen-6(KL-6) ELISA Kit

$458.00 USD
$458.00 USD
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Sensitivity

Human Krebs Von den Lungen-6(KL-6) ELISA Kit

Overview

Product name: Human Krebs Von den Lungen-6(KL-6) ELISA Kit
Reactivity: Human
Alternative Names: KL-6
Assay Type: Competitive Inhibition
Sensitivity: 5.9 IU/mL
Standard: 800 IU/mL
Detection Range: 12.5-800 IU/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 2h
Research Area: Interstitial lung disease
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KL-6 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KL-6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
800.00 0.212 —
400.00 0.411 —
200.00 0.612 —
100.00 0.833 —
50.00 1.157 —
25.00 1.596 —
12.50 1.877 —
0.00 2.101 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant KL-6 and the recovery rates were calculated by comparing the measured value to the expected amount of KL-6 in samples.
Matrix Recovery range Average
serum(n=5) 95-107% 101%
EDTA plasma(n=5) 82-95% 88%
Heparin plasma(n=5) 86-99% 92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of KL-6 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-95% 96-102% 85-100% 97-106%
EDTA plasma(n=5) 86-97% 92-99% 85-94% 81-92%
Heparin plasma(n=5) 85-94% 87-105% 92-101% 79-90%
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