Rat PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit

Name: Rat PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit
Brand: ELK Biotechnology
SKU: ELK9397
Price: 458.0 USD
Availability: InStock

$458.00 USD

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Sensitivity 6.2 ng/mL

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Rat PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit

Overview

Product name:	Rat PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit
Reactivity:	Rat
Alternative Names:	PHOSPHATIDYLINOSITOL GLYCAN; Phosphatidylinositol glycan biosynthesis class F protein; Phosphatidylinositol-glycan biosynthesis class F protein; PIG F; PIG-F; PIGF; PIGF_HUMAN; PIGF1
Assay Type:	Competitive Inhibition
Sensitivity:	6.2 ng/mL
Standard:	1000 ng/mL
Detection Range:	15.6-1000 ng/mL
Sample Type:	Serum, plasma, tissue homogenates and other biological fluids
Assay Length:	2h
Research Area:	Signal transduction;
Test principle:	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat F protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL)	OD	Corrected OD
1000.00	0.217	—
500.00	0.336	—
250.00	0.527	—
125.00	0.899	—
62.50	1.253	—
31.25	1.557	—
15.63	1.796	—
0.00	2.112	—

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant F and the recovery rates were calculated by comparing the measured value to the expected amount of F in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	81-95%	88%
Heparin plasma(n=5)	87-95%	91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of F and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.