| TARGET | |
|---|---|
| SYNONYMS |
CD80;7;7-1;7.1;B1;D28LG;D28LG1;AB7 |
| DESCRIPTION |
Recombinant Human B7-1 Protein with C-terminal Mouse Fc and 6×His tag |
| DELIVERY |
In Stock |
| UNIPROT ID |
P33681 |
| EXPRESSION HOST |
HEK293 |
| TAG |
C-Mouse Fc and 6×His Tag |
| MOLECULAR CHARACTERIZATION |
B7-1(Val35-Asn242) mFc(Pro99-Lys330) 6×His tag |
| MOLECULAR WEIGHT |
The protein has a predicted molecular mass of 50.9 kDa after removal of the signal peptide. The apparent molecular mass of B7-1-mFc-His is approximately 70-130 kDa due to glycosylation. |
| PURITY |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| FORMULATION & RECONSTITUTION |
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. |
| USAGE |
Research use only |
Dima Biotech
SKU:PME100047
Human B7-1 Protein, mFc-His Tag
Human B7-1 Protein, mFc-His Tag
Regular price
$555.00 USD
Regular price
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$555.00 USD
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PRODUCT DATA
IMAGES
Figure 1. Human B7-1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human B7-1, mFc-His tagged protein (PME100047) can bind anti-B7-1 monoclonal antibody,Rabbit mAb (clone: DM111) in a linear range of 0.13-80 ng/ml.
Figure 3. Flow cytometry analysis with 15 μg/mL Human B7-1 Protein, mFc-His tag (PME100047) on Expi293 cells transfected with human CD28 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
