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Dima Biotech

SKU:PME100512

Human LAG3 Protein, His Tag

Human LAG3 Protein, His Tag

Regular price $478.00 USD
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Size
Target
Uniprot ID
PRODUCT DATA
TARGET

SYNONYMS

LAG-3;CD223

DESCRIPTION

Recombinant Human LAG3 Protein with C-terminal 6×His tag

DELIVERY

In Stock

UNIPROT ID

P18627

EXPRESSION HOST

HEK293

TAG

C-6×His Tag

MOLECULAR CHARACTERIZATION

LAG3(Leu23-Leu245) 6×His tag

MOLECULAR WEIGHT

The protein has a predicted molecular mass of 47.0 kDa after removal of the signal peptide. The apparent molecular mass of LAG3-His is approximately 55-70 kDa due to glycosylation.

PURITY

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

FORMULATION & RECONSTITUTION

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

STORAGE & SHIPPING

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

BACKGROUND

Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]

USAGE

Research use only

IMAGES

sp-PME100512 LAG3 His PAGE 300

Figure 1. Human LAG3 Protein, His Tag on SDS-PAGE under reducing condition.

elisa-PME100512 LAG3 His ELISA Fig2

Figure 2. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human LAG3 , His tagged protein (PME100512) can bind Anti-LAG3 Neutralizing antibody (BME100062) in a linear range of 16-80ng/ml.

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