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Dima Biotech

DME100001

Anti-His Tag Antibody(DM1), Rabbit mAb

Anti-His Tag Antibody(DM1), Rabbit mAb

$438.00 USD
$438.00 USD
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Size
Recommended-dilutions
clonality
PRODUCT DATA
TARGET

SYNONYMS

his tag
HOST SPECIES

Rabbit
DESCRIPTION

Anti-His Tag Antibody(DM1); Rabbit mAb
DELIVERY

In Stock
IGG TYPE

Rabbit IgG
CLONALITY

Monoclonal
REACTIVITY

Detects proteins containing accessible consecutive histidine regions. The antibody detects His tags localized at the amino- or carboxyl- terminus.
APPLICATIONS

ELISA; WB
RECOMMENDED DILUTIONS

WB 1:1000
PURIFICATION

Purified from cell culture supernatant by affinity chromatography
FORMULATION & RECONSTITUTION

Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
STORAGE & SHIPPING

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
BACKGROUND

The rabbit immune system generates antibody diversity and optimizes affinity. Anti-His Tag Antibody(DM1); Rabbit mAb is specific to six histidine tags placed at C-terminal and internal regions of fusion proteins. This antibody can greatly improve the effectiveness of several different kinds ofimmunoassays; helping researchers identify; detect; and purify polyhistidine fusion proteinsin bacteria; insect cells; and mammalian cells.
USAGE

Research use only
IMAGES

antibody-DME100001 His Fig1
Western blot analysis of his-tag fusion protein using His-Tag antibody (DME100001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:5000 dilution.
His-tag fusion protein : 70kDa, 20ng per lane.
Blocking buffer: 5% nonfat dry milk in TBST.
Exposure time: 1s.

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