| TARGET | |
|---|---|
| SYNONYMS |
ANKTM1; FEPS; FEPS1 |
| DESCRIPTION |
Human TRPA1 full length protein-synthetic nanodisc |
| DELIVERY |
In Stock |
| UNIPROT ID |
O75762 |
| EXPRESSION HOST |
HEK293 |
| PROTEIN FAMILIES |
Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| PROTEIN PATHWAYS |
—— |
| MOLECULAR WEIGHT |
The human full length TRPA1 protein has a MW of 127.5 kDa |
| FORMULATION & RECONSTITUTION |
Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| STORAGE & SHIPPING |
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| BACKGROUND |
The structure of the protein is highly related to both the protein ankyrin and transmembrane proteins. This protein is activated by a large variety of structurally unrelated electrophilic and non-electrophilic chemical compounds. Electrophilic ligands activate TRPA1 by interacting with critical N-terminal Cys residues in a covalent manner, whereas mechanisms of non-electrophilic ligands are not well determined. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system. |
| USAGE |
Research use only |
Dima Biotech
SKU:FLP100033
Human TRPA1 full length protein-synthetic nanodisc
Human TRPA1 full length protein-synthetic nanodisc
Regular price
$900.00 USD
Regular price
Sale price
$900.00 USD
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PRODUCT DATA
IMAGES
Figure1. Elisa plates were pre-coated with Flag Tag TRPA1-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with TRPA1-Nanodisc is 7.433ng/ml.
Figure2. Human TRPA1-Nanodisc, Flag Tag on SDS-PAGE
