NET (Phospho-S357) polyclonal antibody

Numerous cellular functions, such as proliferation, differentiation, apoptosis, vesicular trafficking, nuclear transport and cytoskeletal organization, are controlled by GTPases. It has become increasingly clear that GTPases act in cascades in which their activities are linked by GTPase-activating proteins (GAPs) and guanine nucleotide exchange factors (GEFs). Researchers looking for new epithelial cell-specific oncogenes, using a highly efficient cDNA expression cloning system, have isolated the Ost oncogene from rat osteosarcoma cells. The Ost proto-oncogene protein contains DH and PH domains, catalyzes guanine nucleotide exchange on Rho A and Cdc42, and interacts specifically with the GTP-bound form of Rac1. The related NET1 protein also contains a DH domain and is ubiquitously expressed in a variety of tissues. Overexpression of NET1 in NIH/3T3 cells results in altered growth properties and tumorigenesis when injected into nude mice.