AF647 Anti-Mouse CD4 Antibody[GK1.5]

AF647 Anti-Mouse CD4 Antibody[GK1.5]

AF647 Anti-Mouse CD4 Antibody[GK1.5] is a highly specific monoclonal antibody that recognizes mouse CD4, a cell surface glycoprotein expressed on T helper cells, regulatory T cells, and thymocytes. This antibody is conjugated with AF647, a bright and photostable fluorescent dye that emits in the far-red region of the spectrum, making it suitable for flow cytometry, microscopy, and other imaging applications. The GK1.5 clone has been extensively validated and widely used in research to study T cell development, activation, differentiation, and function.

This antibody has been purified from hybridoma culture supernatant by protein A affinity chromatography and formulated in phosphate-buffered saline (PBS) with 0.1% sodium azide as a preservative. It has a high degree of purity and specificity, with minimal cross-reactivity to other mouse antigens. The recommended working concentration for flow cytometry is 0.25-1 µg per million cells in 100 µl staining buffer. For microscopy, the optimal dilution should be determined by the user based on the specific application and sample type.

AF647 Anti-Mouse CD4 Antibody[GK1.5] is a valuable tool for researchers studying the immune system, autoimmune diseases, infectious diseases, cancer, and other fields where T cells play a critical role. Its bright and stable fluorescence, combined with its high specificity and purity, make it an ideal choice for multiparameter flow cytometry and imaging experiments. With its well-established performance and reliability, this antibody is a trusted reagent for many researchers worldwide.