Human AP13(Apelin 13) ELISA Kit

The Human AP13 (Apelin 13) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative measurement of Apelin 13 in human serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes two specific antibodies that bind to different epitopes of Apelin 13. The assay is performed in a 96-well microplate coated with a monoclonal antibody against Apelin 13. The sample is added to the wells, and the Apelin 13 present in the sample binds to the immobilized antibody.

After washing away any unbound substances, a biotinylated polyclonal antibody specific to Apelin 13 is added to the wells, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes the conversion of the substrate, resulting in a color change that is proportional to the amount of Apelin 13 present in the sample. The absorbance is measured at 450 nm using a microplate reader. This kit provides a simple, accurate, and reproducible method for the detection of Apelin 13 in human samples, making it an essential tool for research and clinical applications.