Human EGF(Epidermal Growth Factor) ELISA Kit

The Human EGF (Epidermal Growth Factor) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the quantitative measurement of human EGF in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes two specific antibodies that bind to different epitopes of the target protein.

The assay is performed in a 96-well microplate coated with a monoclonal antibody against human EGF. The sample is added to the wells, and the EGF present in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated polyclonal antibody specific to human EGF is added to the wells, which binds to the captured EGF. Following another washing step, streptavidin-conjugated horseradish peroxidase is added to the wells, which binds to the biotinylated antibody. Finally, a substrate solution is added, and the color development is measured using a microplate reader. The concentration of human EGF in the sample is determined by comparing the optical density of the sample to a standard curve. This kit is highly sensitive, with a detection range of 7.8-500 pg/mL, and has a high degree of reproducibility and accuracy. It is an essential tool for researchers and clinicians studying the role of EGF in various physiological and pathological processes.