Human ET-1(Endothelin 1) ELISA Kit

The Human ET-1 (Endothelin 1) ELISA Kit is a highly reliable and sensitive diagnostic tool designed for the quantitative detection of Endothelin 1 in human serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes monoclonal antibodies specific to human ET-1. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human ET-1. The sample is added to the wells, and the ET-1 present in the sample binds to the immobilized antibody.

After washing away any unbound substances, a biotinylated monoclonal antibody specific to human ET-1 is added to the wells. This antibody binds to the ET-1 captured by the first antibody. Following another washing step, streptavidin-conjugated horseradish peroxidase (HRP) is added to the wells. The HRP catalyzes the conversion of the substrate, resulting in a color change that is proportional to the amount of ET-1 present in the sample. The Human ET-1 ELISA Kit is a highly accurate and precise diagnostic tool that provides reliable results in a short period of time.