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Elabscience
SKU:E-EL-H0070
Human αFP(Alpha-Fetoprotein) ELISA Kit
Human αFP(Alpha-Fetoprotein) ELISA Kit
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$495.00 USD
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The Human AFP (Alpha-Fetoprotein) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the quantitative measurement of AFP in human serum or plasma samples. This kit is intended for use in clinical laboratories and research settings to aid in the diagnosis and monitoring of various diseases, including liver cancer, germ cell tumors, and fetal abnormalities.
The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which involves the use of two specific antibodies that bind to different epitopes of AFP. The first antibody is coated onto the microplate wells, while the second antibody is conjugated to horseradish peroxidase (HRP). The sample is added to the wells, and AFP in the sample binds to the first antibody. The HRP-conjugated second antibody is then added, which binds to the AFP, forming a sandwich complex. The addition of a substrate solution causes a color change, which is proportional to the amount of AFP present in the sample.
The Human AFP ELISA Kit is easy to use, with a simple and straightforward protocol that can be completed in less than 3 hours. The kit has a high sensitivity and specificity, with a detection range of 0.5-200 ng/mL. It is also highly reproducible, with a coefficient of variation (CV) of less than 10%. The kit is supplied with all necessary reagents and materials, including a microplate, standards, controls, and a detailed instruction manual.
The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) technique, which involves the use of two specific antibodies that bind to different epitopes of AFP. The first antibody is coated onto the microplate wells, while the second antibody is conjugated to horseradish peroxidase (HRP). The sample is added to the wells, and AFP in the sample binds to the first antibody. The HRP-conjugated second antibody is then added, which binds to the AFP, forming a sandwich complex. The addition of a substrate solution causes a color change, which is proportional to the amount of AFP present in the sample.
The Human AFP ELISA Kit is easy to use, with a simple and straightforward protocol that can be completed in less than 3 hours. The kit has a high sensitivity and specificity, with a detection range of 0.5-200 ng/mL. It is also highly reproducible, with a coefficient of variation (CV) of less than 10%. The kit is supplied with all necessary reagents and materials, including a microplate, standards, controls, and a detailed instruction manual.
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