Human G-CSF(Granulocyte Colony-stimulating Factor) ELISA Kit

The Human G-CSF (Granulocyte Colony-stimulating Factor) ELISA Kit is a highly reliable and sensitive diagnostic tool designed for the quantitative detection of human G-CSF in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes two specific antibodies that bind to different epitopes of the target protein.

The assay is performed in a 96-well microplate coated with a monoclonal antibody against human G-CSF. The sample is added to the wells, and the G-CSF in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated polyclonal antibody specific to human G-CSF is added to the wells, which binds to the captured G-CSF. Following another washing step, streptavidin-conjugated horseradish peroxidase (HRP) is added to the wells, which binds to the biotinylated antibody. Finally, a substrate solution is added, and the color development is measured using a microplate reader. The Human G-CSF ELISA Kit is a valuable tool for researchers and clinicians in the fields of immunology, oncology, and hematology, providing accurate and precise quantification of human G-CSF levels in various biological samples.