Human GDF15(Growth Differentiation Factor 15) ELISA Kit

The Human GDF15 (Growth Differentiation Factor 15) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative measurement of GDF15 in human serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of GDF15. The assay is performed in a 96-well microplate coated with a monoclonal antibody against GDF15. The sample is added to the wells, and the GDF15 present in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated polyclonal antibody specific for GDF15 is added to the wells. Following another washing step, streptavidin-conjugated horseradish peroxidase (HRP) is added to the wells, which binds to the biotinylated antibody.

Finally, a substrate solution is added to the wells, and the color development is measured using a microplate reader. The concentration of GDF15 in the sample is determined by comparing the optical density of the sample to a standard curve. This kit is highly specific, accurate, and reproducible, making it an ideal tool for research and clinical applications.