Human IGF-1(Insulin-like Growth Factor 1) ELISA Kit

The Human IGF-1 (Insulin-like Growth Factor 1) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative measurement of human IGF-1 in serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes two specific antibodies that bind to different epitopes of the target protein.

The assay is performed in a 96-well microplate coated with a monoclonal antibody against human IGF-1. The sample is added to the plate, and the IGF-1 in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated polyclonal antibody specific to human IGF-1 is added to the plate, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes the conversion of the substrate, resulting in a color change that is proportional to the amount of IGF-1 present in the sample. The kit has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. This kit is intended for research use only and is not for diagnostic or therapeutic purposes.