Human FGF7/KGF(Fibroblast Growth Factor 7) ELISA Kit

The Human FGF7/KGF (Fibroblast Growth Factor 7) ELISA Kit is a highly sensitive and specific assay designed to measure the levels of FGF7/KGF in human serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that recognize different epitopes of FGF7/KGF. The capture antibody is coated onto a microplate, and the detection antibody is conjugated to horseradish peroxidase (HRP). The assay is performed by adding the sample to the microplate, followed by the detection antibody-HRP conjugate. After washing, a substrate solution is added, and the reaction is stopped with an acidic solution. The absorbance is measured at 450 nm, and the concentration of FGF7/KGF in the sample is determined by comparing the absorbance to a standard curve.

FGF7/KGF is a member of the fibroblast growth factor (FGF) family, which plays a crucial role in cell proliferation, differentiation, and survival. FGF7/KGF is mainly produced by mesenchymal cells and acts on epithelial cells, promoting their growth and differentiation. FGF7/KGF is involved in various physiological processes, such as wound healing, tissue repair, and development of the lung, skin, and hair follicles. Abnormal levels of FGF7/KGF have been associated with several diseases, including cancer, pulmonary fibrosis, and skin disorders.

The Human FGF7/KGF ELISA Kit is a reliable and accurate tool for the quantitative measurement of FGF7/KGF in human samples. This kit has a detection range of 15.6-1000 pg/mL, with a sensitivity of 7.8 pg/mL. The assay is easy to perform, with a total assay time of 3 hours and 30 minutes. The kit includes all the necessary reagents and a detailed protocol, making it suitable for both novice and experienced researchers. The Human FGF7/KGF ELISA Kit is an essential tool for studying the role of FGF7/KGF in various physiological and pathological conditions.