Human ISG15(Interferon Stimulated Gene 15) ELISA Kit

The Human ISG15 (Interferon Stimulated Gene 15) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human ISG15 in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes a monoclonal antibody specific to human ISG15 coated onto a microplate.

The sample is added to the microplate, and any human ISG15 present in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated monoclonal antibody specific to human ISG15 is added to the microplate, which binds to the captured human ISG15. Following another washing step, streptavidin-conjugated horseradish peroxidase is added to the microplate, which binds to the biotinylated antibody. Finally, a substrate solution is added to the microplate, and the color development is measured at 450 nm. The Human ISG15 ELISA Kit is a valuable tool for researchers studying the role of ISG15 in various biological processes, including immune response, viral infection, and cancer.