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Elabscience
SKU:E-EL-H0346
Human CNN1(Calponin 1, Basic, Smooth Muscle) ELISA Kit
Human CNN1(Calponin 1, Basic, Smooth Muscle) ELISA Kit
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The Human CNN1 (Calponin 1, Basic, Smooth Muscle) ELISA Kit is a highly reliable and accurate tool designed for the quantitative measurement of Calponin 1 in human samples. Calponin 1, also known as CNN1, is a protein that plays a crucial role in the regulation of smooth muscle contraction. This ELISA kit utilizes a sandwich enzyme immunoassay technique, ensuring high sensitivity and specificity.
The kit includes all the necessary components for the assay, including pre-coated microplates, standards, controls, and reagents. The microplates are pre-coated with a specific antibody against Calponin 1, ensuring efficient capture of the target protein. The standards and controls provided allow for the generation of a standard curve, enabling accurate quantification of Calponin 1 levels in the samples.
The assay procedure is simple and straightforward, with a total assay time of approximately 3 hours. The samples, standards, and controls are added to the microplate wells, followed by the addition of a biotin-conjugated antibody specific to Calponin 1. After a brief incubation, the wells are washed to remove any unbound substances. Streptavidin-HRP is then added, which binds to the biotin-conjugated antibody. Following another washing step, a substrate solution is added, resulting in the development of a blue color. The intensity of the color is directly proportional to the amount of Calponin 1 present in the samples. Finally, the reaction is stopped, and the absorbance is measured at a specific wavelength using a microplate reader.
The Human CNN1 ELISA Kit provides researchers and clinicians with a reliable and efficient tool for the quantitative determination of Calponin 1 levels in human samples. Its high sensitivity, specificity, and ease of use make it an invaluable asset in various research and diagnostic applications.
The kit includes all the necessary components for the assay, including pre-coated microplates, standards, controls, and reagents. The microplates are pre-coated with a specific antibody against Calponin 1, ensuring efficient capture of the target protein. The standards and controls provided allow for the generation of a standard curve, enabling accurate quantification of Calponin 1 levels in the samples.
The assay procedure is simple and straightforward, with a total assay time of approximately 3 hours. The samples, standards, and controls are added to the microplate wells, followed by the addition of a biotin-conjugated antibody specific to Calponin 1. After a brief incubation, the wells are washed to remove any unbound substances. Streptavidin-HRP is then added, which binds to the biotin-conjugated antibody. Following another washing step, a substrate solution is added, resulting in the development of a blue color. The intensity of the color is directly proportional to the amount of Calponin 1 present in the samples. Finally, the reaction is stopped, and the absorbance is measured at a specific wavelength using a microplate reader.
The Human CNN1 ELISA Kit provides researchers and clinicians with a reliable and efficient tool for the quantitative determination of Calponin 1 levels in human samples. Its high sensitivity, specificity, and ease of use make it an invaluable asset in various research and diagnostic applications.
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