Human FASN(Fatty Acid Synthase) ELISA Kit

The Human FASN (Fatty Acid Synthase) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of FASN in human serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that recognize different epitopes of FASN. The capture antibody is coated onto the microplate wells, while the detection antibody is conjugated to horseradish peroxidase (HRP). The FASN standard or samples are added to the wells, and the FASN in the sample binds to the capture antibody. After washing away any unbound substances, the HRP-conjugated detection antibody is added, which binds to the FASN captured by the capture antibody.

The excess detection antibody is washed away, and a substrate solution is added, which reacts with the HRP to produce a color change. The intensity of the color is directly proportional to the amount of FASN present in the sample. This kit is easy to use, highly specific, and has a wide dynamic range, making it an ideal tool for researchers studying FASN in human samples.