Human GC(Glycocalicin) ELISA Kit

The Human GC (Glycocalicin) ELISA Kit is a highly reliable and sensitive diagnostic tool designed for the quantitative detection of human GC in serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human GC. The sample is added to the wells, and the target protein binds to the immobilized antibody.

After washing, a biotinylated polyclonal antibody against human GC is added, which binds to the captured protein. The plate is then washed again, and a streptavidin-horseradish peroxidase conjugate is added, which binds to the biotinylated antibody. Finally, a chromogenic substrate is added, and the color intensity is measured using a microplate reader. The concentration of human GC in the sample is determined by comparing the absorbance values to a standard curve. This kit is easy to use, highly specific, and has a wide dynamic range, making it an ideal tool for clinical and research applications.