Human AQP-2(Aquaporin 2) ELISA Kit

The Human AQP-2 (Aquaporin 2) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human AQP-2 in biological samples. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein.

The assay is performed in a 96-well microplate coated with a monoclonal antibody against human AQP-2. The sample is added to the wells, and the target protein binds to the immobilized antibody. After washing, a biotinylated polyclonal antibody against human AQP-2 is added, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes the conversion of the substrate into a colored product, which is proportional to the amount of human AQP-2 present in the sample. The absorbance is measured at 450 nm using a microplate reader. This kit has a detection range of 0.156-10 ng/mL and a sensitivity of 0.094 ng/mL. It is suitable for use in research and clinical laboratories for the diagnosis and monitoring of human AQP-2-related diseases.