Human CAM(Calmodulin) ELISA Kit

The Human CAM (Calmodulin) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human Calmodulin in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes a monoclonal antibody specific to human Calmodulin coated onto a microplate. The sample is added to the microplate, and any Calmodulin present in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated monoclonal antibody specific to human Calmodulin is added to the microplate. This antibody binds to the Calmodulin captured during the first incubation. Following another washing step, streptavidin-conjugated horseradish peroxidase (HRP) is added to the microplate.

This binds to the biotinylated antibody, and the resulting complex is detected by the addition of a chromogenic substrate. The intensity of the color generated is proportional to the amount of Calmodulin present in the sample. This kit is highly specific, with no cross-reactivity with other proteins. It is easy to use, with a short assay time of only 2.5 hours. The Human CAM (Calmodulin) ELISA Kit is an essential tool for researchers studying the role of Calmodulin in various physiological and pathological processes.