Human CASP4(Caspase 4) ELISA Kit

The Human CASP4 (Caspase 4) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human CASP4 in various biological samples. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human CASP4. The sample is added to the wells, and the target protein binds to the immobilized antibody. After washing, a biotinylated polyclonal antibody against human CASP4 is added, which binds to the captured protein.

Streptavidin-HRP conjugate is then added, which binds to the biotinylated antibody. Finally, a substrate solution is added, and the color development is measured using a microplate reader. The concentration of human CASP4 in the sample is determined by comparing the absorbance of the sample to a standard curve. This kit has a detection range of 0.156-10 ng/mL and a sensitivity of 0.094 ng/mL. It is suitable for research use in various fields, including cancer, inflammation, and apoptosis.