Human CAD(Caspase Activated Deoxyribonuclease) ELISA Kit

The Human CAD (Caspase Activated Deoxyribonuclease) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the detection of CAD in human serum, plasma, and cell culture supernatants. This kit is based on the principle of sandwich ELISA, which utilizes two specific antibodies that bind to different epitopes of CAD. The first antibody is coated onto the microplate wells, while the second antibody is conjugated to horseradish peroxidase (HRP). The sample is added to the wells, and CAD present in the sample binds to the first antibody.

The second antibody-HRP conjugate is then added, which binds to the CAD-antibody complex, resulting in the formation of a sandwich. The unbound components are washed away, and a substrate solution is added, which reacts with the HRP enzyme to produce a colorimetric signal that is directly proportional to the amount of CAD present in the sample. The Human CAD ELISA Kit is highly sensitive, with a detection range of 0.156-10 ng/mL, and has a low coefficient of variation (CV) of less than 10%. This kit is easy to use, with a simple protocol that can be completed in less than 3 hours. It is an essential tool for researchers and clinicians involved in the study and diagnosis of CAD-related diseases.