Human CCL16(Chemokine C-C-Motif Ligand 16) ELISA Kit

The Human CCL16 (Chemokine C-C-Motif Ligand 16) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human CCL16 in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes a monoclonal antibody specific to human CCL16 coated onto a microplate. The sample is added to the microplate, and any CCL16 present in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated monoclonal antibody specific to human CCL16 is added to the microplate, which binds to the captured CCL16.

Following another washing step, streptavidin-conjugated horseradish peroxidase is added to the microplate, which binds to the biotinylated antibody. Finally, a substrate solution is added to the microplate, which reacts with the bound enzyme to produce a colorimetric signal that is proportional to the amount of CCL16 present in the sample. This kit is highly specific, with no cross-reactivity observed with other human cytokines or chemokines. The Human CCL16 ELISA Kit is a valuable tool for researchers studying the role of CCL16 in various physiological and pathological processes.