Human CBX3(Chromobox Homolog 3) ELISA Kit

The Human CBX3 (Chromobox Homolog 3) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of CBX3 in human serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of CBX3. The assay is performed in a 96-well microplate coated with a monoclonal antibody specific to CBX3.

The sample is added to the wells, and CBX3 present in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated polyclonal antibody specific to CBX3 is added to the wells, which binds to the captured CBX3. Following another washing step, streptavidin-conjugated horseradish peroxidase (HRP) is added to the wells, which binds to the biotinylated antibody. Finally, a substrate solution is added to the wells, and the color development is measured using a microplate reader. The concentration of CBX3 in the sample is determined by comparing the optical density of the sample to the standard curve. This kit provides a simple, accurate, and reproducible method for the detection of CBX3 in human samples.