Human COL2α1(Collagen Type Ⅱ Alpha 1) ELISA Kit

The Human COL2Α1 (Collagen Type Ⅱ Alpha 1) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative determination of human COL2Α1 in serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human COL2Α1.

The sample is added to the wells, and the target protein binds to the immobilized antibody. After washing away unbound substances, a biotinylated polyclonal antibody against human COL2Α1 is added, which binds to the captured protein. Streptavidin-HRP conjugate is then added, which binds to the biotinylated antibody. Finally, a substrate solution is added, and the color development is measured at 450 nm. The concentration of human COL2Α1 in the sample is determined by comparing the optical density of the sample to the standard curve. This kit is highly specific, with no cross-reactivity with other human collagens. It is easy to use, with a short assay time of 2.5 hours, and has a detection range of 0.156-10 ng/mL. This kit is ideal for research and clinical applications, including the diagnosis and monitoring of osteoarthritis and other collagen-related diseases.