Human COL11α1(Collagen Type Ⅺ Alpha 1) ELISA Kit

The Human COL11Α1 (Collagen Type Ⅺ Alpha 1) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human COL11Α1 in serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human COL11Α1.

The sample is added to the wells, and the target protein binds to the immobilized antibody. After washing away unbound substances, a biotinylated polyclonal antibody against human COL11Α1 is added, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes the conversion of the chromogenic substrate, resulting in a color change that is proportional to the amount of human COL11Α1 present in the sample. The absorbance is measured at 450 nm using a microplate reader. This kit has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.094 ng/mL. It is a valuable tool for researchers and clinicians studying the role of COL11Α1 in various diseases and conditions.