Human COL17(Collagen Type ⅩⅦ) ELISA Kit

The Human COL17 (Collagen Type ⅩⅦ) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human COL17 in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human COL17.

The sample is added to the wells, and the target protein binds to the immobilized antibody. After washing away unbound substances, a biotinylated polyclonal antibody against human COL17 is added, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes the conversion of the chromogenic substrate, resulting in a color change that is proportional to the amount of human COL17 present in the sample. The assay has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. This kit is intended for research use only and is not for diagnostic or therapeutic purposes. It is a valuable tool for studying the role of human COL17 in various physiological and pathological processes.