Human TLR9(Toll-Like Receptor 9) ELISA Kit

The Human TLR9 (Toll-Like Receptor 9) ELISA Kit is a highly sensitive and specific assay designed to detect and quantify TLR9 protein levels in human serum, plasma, and cell culture supernatants. Toll-like receptors (TLRs) are a family of pattern recognition receptors that play a critical role in the innate immune response to microbial pathogens. TLR9 is specifically involved in the recognition of unmethylated CpG DNA motifs found in bacterial and viral genomes, as well as in synthetic oligonucleotides used in immunotherapy.

The ELISA kit utilizes a sandwich enzyme-linked immunosorbent assay format, in which TLR9 protein is captured by a monoclonal antibody coated on a microplate, and then detected by a biotinylated polyclonal antibody and streptavidin-conjugated horseradish peroxidase. The assay has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. The kit includes all necessary reagents and controls, and can be used for both qualitative and quantitative analysis of TLR9 protein levels.

The Human TLR9 ELISA Kit is a valuable tool for researchers studying the role of TLR9 in infectious diseases, cancer, and autoimmune disorders. It can be used to monitor TLR9 expression levels in patient samples, as well as in vitro models of immune cell activation and response. The kit is easy to use, with a simple protocol that can be completed in less than 4 hours. It is also highly reproducible, with low intra- and inter-assay variability. Overall, the Human TLR9 ELISA Kit is a reliable and accurate method for measuring TLR9 protein levels in human samples.