Human GAL8(Galectin 8) ELISA Kit

The Human GAL8 (Galectin 8) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human GAL8 in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human GAL8. The sample is added to the wells, and the target protein is captured by the immobilized antibody.

After washing away unbound substances, a biotinylated polyclonal antibody against human GAL8 is added, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes the conversion of the chromogenic substrate, resulting in a color change that is proportional to the amount of human GAL8 present in the sample. The assay has a detection range of 0.156-10 ng/mL, with a sensitivity of 0.078 ng/mL. This kit is easy to use, with a short assay time of 3 hours, and requires minimal sample preparation. It is suitable for research and clinical applications, providing accurate and reproducible results.