Human FGF9(Fibroblast Growth Factor 9) ELISA Kit

The Human FGF9 (Fibroblast Growth Factor 9) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human FGF9 in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human FGF9.

The sample is added to the wells, and the target protein binds to the immobilized antibody. After washing, a biotinylated polyclonal antibody against human FGF9 is added, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes the conversion of the chromogenic substrate, resulting in a color change that is proportional to the amount of human FGF9 present in the sample. The kit has a detection range of 15.6-1000 pg/mL, with a sensitivity of 9.4 pg/mL. The Human FGF9 ELISA Kit is a valuable tool for researchers studying the role of FGF9 in various biological processes, including development, tissue repair, and cancer.