Human MIF(Macrophage Migration Inhibitory Factor) ELISA Kit

The Human MIF (Macrophage Migration Inhibitory Factor) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the quantitative detection of human MIF in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes monoclonal antibodies specific to human MIF. The assay is performed in a 96-well microplate coated with a capture antibody that binds to human MIF. The sample is then added to the microplate, and any human MIF present in the sample binds to the capture antibody.

A detection antibody conjugated to horseradish peroxidase is then added, which binds to the captured human MIF. The resulting complex is detected by the addition of a substrate solution, which produces a colorimetric signal that is proportional to the amount of human MIF present in the sample. This kit is highly sensitive, with a detection range of 0.156-10 ng/mL, and has a low coefficient of variation (CV) of less than 10%. It is suitable for use in research and clinical laboratories for the diagnosis and monitoring of various diseases associated with human MIF.