Human GPC3(Glypican 3) ELISA Kit

The Human GPC3 (Glypican 3) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of GPC3 in human serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that recognize different epitopes of GPC3. The capture antibody is coated onto the microplate wells, while the detection antibody is conjugated to horseradish peroxidase (HRP). The assay is performed by adding the sample and the detection antibody to the wells, followed by incubation and washing steps. The HRP substrate is then added, and the color development is measured using a microplate reader.

GPC3 is a glycosylphosphatidylinositol (GPI)-anchored protein that is highly expressed in hepatocellular carcinoma (HCC) and is considered a potential biomarker for HCC diagnosis and prognosis. The Human GPC3 ELISA Kit provides a reliable and accurate method for the detection of GPC3 in human samples, with a detection range of 0.156-10 ng/mL. The kit has a high sensitivity, with a minimum detectable dose of 0.039 ng/mL, and a low intra-assay and inter-assay coefficient of variation (CV) of less than 10%. The kit is easy to use, with a simple protocol that can be completed within 3 hours.

The Human GPC3 ELISA Kit is intended for research use only and is not for diagnostic or therapeutic purposes. The kit includes all the necessary components for the assay, including microplate wells, capture and detection antibodies, HRP-conjugated detection antibody, assay buffer, substrate solution, and stop solution. The kit has been validated for human samples, but it may also be used for the detection of GPC3 in other species with appropriate sample preparation and optimization. The kit is manufactured in compliance with ISO 13485 quality management system and is CE marked.