Human Grb10(Growth Factor Receptor Bound Protein 10) ELISA Kit

The Human Grb10 (Growth Factor Receptor Bound Protein 10) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of Grb10 in human serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of Grb10. The captured Grb10 is then detected using a biotinylated detection antibody and streptavidin-HRP conjugate, followed by the addition of a chromogenic substrate that produces a colorimetric signal proportional to the amount of Grb10 present in the sample.

This ELISA kit has a wide dynamic range of detection, with a lower limit of detection of 0.156 ng/mL and an upper limit of 10 ng/mL. It has been validated for its accuracy, precision, and specificity, ensuring reliable and reproducible results. The kit is easy to use, with a simple protocol that can be completed within 3 hours. It is suitable for research applications in the fields of cancer, diabetes, and obesity, where Grb10 has been implicated in the regulation of cell growth, metabolism, and insulin signaling.