Human CK-17/KRT17(Cytokeratin 17) ELISA Kit

The Human CK-17/KRT17 (Cytokeratin 17) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human CK-17/KRT17 in serum, plasma, and other biological fluids. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of CK-17/KRT17. The assay is performed in a 96-well microplate coated with a monoclonal antibody against CK-17/KRT17. The sample is added to the wells, and the CK-17/KRT17 in the sample binds to the immobilized antibody.

After washing away any unbound substances, a biotinylated polyclonal antibody specific for CK-17/KRT17 is added to the wells. Following another washing step, streptavidin-conjugated horseradish peroxidase (HRP) is added to the wells, which binds to the biotinylated antibody. Finally, a substrate solution is added to the wells, and the color development is measured using a microplate reader. The concentration of CK-17/KRT17 in the sample is determined by comparing the optical density of the sample to a standard curve. This kit is highly specific, accurate, and reproducible, making it an ideal tool for research and clinical applications.