Human CK-20/KRT20(Cytokeratin 20) ELISA Kit

The Human CK-20/KRT20 (Cytokeratin 20) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human CK-20/KRT20 in various biological samples. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of CK-20/KRT20. The assay is performed in a 96-well microplate coated with a monoclonal antibody against CK-20/KRT20.

The sample is added to the wells, and the CK-20/KRT20 in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated polyclonal antibody specific to CK-20/KRT20 is added to the wells. This antibody binds to the captured CK-20/KRT20, forming a sandwich complex. The complex is then detected by the addition of streptavidin-conjugated horseradish peroxidase (HRP) and subsequent addition of a chromogenic substrate. The intensity of the color produced is proportional to the amount of CK-20/KRT20 present in the sample. This kit is highly specific, with no cross-reactivity with other related proteins. It is suitable for use in research and clinical laboratories for the diagnosis and monitoring of various diseases, including colorectal cancer and bladder cancer.