Human FPN(Ferroportin) ELISA Kit

The Human FPN (Ferroportin) ELISA Kit is a highly reliable and accurate diagnostic tool designed for the quantitative measurement of Ferroportin in human serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique, which utilizes two specific antibodies that bind to different epitopes of Ferroportin. The assay is performed in a 96-well microplate coated with a monoclonal antibody against Ferroportin.

The sample is added to the wells, and the Ferroportin present in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated polyclonal antibody specific to Ferroportin is added to the wells. Following another washing step, streptavidin-HRP conjugate is added to the wells, which binds to the biotinylated antibody. Finally, a substrate solution is added to the wells, and the color development is measured using a microplate reader. The concentration of Ferroportin in the sample is determined by comparing the optical density of the sample to the standard curve. This kit is highly sensitive, with a detection range of 0.156 ng/mL to 10 ng/mL, and has a high degree of reproducibility and accuracy. It is an essential tool for researchers and clinicians studying Ferroportin-related diseases.