Human FGF10(Fibroblast Growth Factor 10) ELISA Kit

The Human FGF10 (Fibroblast Growth Factor 10) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human FGF10 in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human FGF10. The sample is added to the wells, and the target protein binds to the immobilized antibody.

After washing away any unbound substances, a biotinylated polyclonal antibody against human FGF10 is added, followed by streptavidin-conjugated horseradish peroxidase (HRP). The HRP catalyzes the conversion of a colorless substrate to a colored product, which is proportional to the amount of human FGF10 present in the sample. The absorbance is measured at 450 nm using a microplate reader. This kit has a detection range of 15.6-1000 pg/mL and a sensitivity of 9.4 pg/mL. It is a valuable tool for researchers studying the role of FGF10 in various biological processes and diseases.