Human Hepc25(Hepcidin 25) ELISA Kit

The Human HEPC25 (Hepcidin 25) ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of Hepcidin 25 in human serum, plasma, and urine samples. Hepcidin 25 is a small peptide hormone that plays a crucial role in iron metabolism and regulation. It is synthesized and secreted by the liver and acts as a negative regulator of iron absorption and recycling. The measurement of Hepcidin 25 levels is important in the diagnosis and management of iron-related disorders such as anemia, hemochromatosis, and chronic kidney disease.

The kit utilizes a sandwich enzyme-linked immunosorbent assay (ELISA) format, which involves the use of two specific antibodies that recognize different epitopes of Hepcidin 25. The assay is performed in a 96-well microplate coated with a capture antibody that binds to Hepcidin 25 in the sample. After washing away unbound materials, a detection antibody conjugated to horseradish peroxidase (HRP) is added, which binds to the captured Hepcidin 25. The addition of a substrate solution containing HRP substrate tetramethylbenzidine (TMB) results in the development of a blue color, which turns yellow upon the addition of a stop solution. The intensity of the yellow color is proportional to the amount of Hepcidin 25 present in the sample and can be measured using a microplate reader.

The Human HEPC25 (Hepcidin 25) ELISA Kit is a reliable and easy-to-use tool for the quantitative measurement of Hepcidin 25 in human serum, plasma, and urine samples. The kit has a detection range of 0.156-10 ng/mL and a sensitivity of 0.078 ng/mL. It has been validated for use in research and clinical laboratories and provides accurate and reproducible results. The kit includes all necessary reagents and a detailed protocol for performing the assay. It is suitable for use in a variety of applications, including basic research, drug development, and clinical diagnostics.