Human ATRN(Attractin) ELISA Kit

The Human ATRN (Attractin) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative measurement of human ATRN in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of the target protein. The assay is performed in a 96-well microplate coated with a monoclonal antibody against human ATRN.

The sample is added to the wells, and the target protein binds to the immobilized antibody. After washing, a biotinylated polyclonal antibody against human ATRN is added, which binds to the captured protein. The plate is then washed again, and a streptavidin-horseradish peroxidase conjugate is added, which binds to the biotinylated antibody. Finally, a chromogenic substrate is added, and the color intensity is measured using a microplate reader. The concentration of human ATRN in the sample is determined by comparing the absorbance values to a standard curve generated using known concentrations of recombinant human ATRN. This kit is easy to use, highly specific, and has a detection range of 0.156-10 ng/mL. It is suitable for research and diagnostic applications.