Human CD5L(CD5 Antigen-like) ELISA Kit

The Human CD5L (CD5 Antigen-like) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative determination of CD5L in human serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes two specific antibodies that bind to different epitopes of CD5L. The assay is performed in a 96-well microplate coated with a monoclonal antibody specific to CD5L. The sample is added to the wells, and CD5L in the sample binds to the immobilized antibody.

After washing away any unbound substances, a biotinylated polyclonal antibody specific to CD5L is added to the wells, which binds to the captured CD5L. Streptavidin-HRP conjugate is then added, followed by the substrate solution, which produces a color reaction that is proportional to the amount of CD5L present in the sample. The absorbance of each well is measured using a microplate reader, and the concentration of CD5L in the sample is calculated based on a standard curve. This kit is easy to use, highly specific, and provides accurate and reproducible results.