Human FGF3(Fibroblast Growth Factor 3) ELISA Kit

The Human FGF3 (Fibroblast Growth Factor 3) ELISA Kit is a highly reliable and sensitive assay designed for the quantitative detection of human FGF3 in serum, plasma, and cell culture supernatants. This kit is based on the sandwich enzyme-linked immunosorbent assay (ELISA) principle, which utilizes a monoclonal antibody specific to human FGF3 coated onto a microplate.

The sample is added to the microplate, and any FGF3 present in the sample binds to the immobilized antibody. After washing away any unbound substances, a biotinylated monoclonal antibody specific to human FGF3 is added to the microplate, which binds to the captured FGF3. Following another washing step, streptavidin-conjugated horseradish peroxidase is added to the microplate, which binds to the biotinylated antibody. Finally, a substrate solution is added to the microplate, which reacts with the bound enzyme to produce a colorimetric signal that is proportional to the amount of FGF3 present in the sample. This kit is highly specific, with no cross-reactivity with other human FGF family members. It is easy to use, with a short assay time of only 3 hours, making it an ideal tool for researchers and clinicians studying human FGF3.